Coastal upwelling regions associated with low-O2 or ODZ waters represent the largest oceanic N2O fluxes to the atmosphere. We collected a time-series of N2O concentrations in the water-column of the Benguela upwelling system off Africa in the Atlantic Ocean following an upwelling event in November 2016 and in February and August 2017 (collaboration with Dr. Sarah Fawcett, University of Cape Town, South Africa). These measurements will allow evaluating the temporal variability of N2O fluxes to the atmosphere for this region. We will also investigate the modes of N2O production using stable isotopes and isotopomers.